Cup holders Buganda made their way through the group stages after defeating Lango province 1-0 away in Lira of the FUFA Drum.
Acholi and Bukedi also confirmed places in the knockout phase after posting positive results over the weekend.
Vianne Sekajugo was the hero for Buganda with a lone strike in the 18th minute. Buganda now leads group B with 12 points from 5 games.
Sekajugo was named Man of the Match.
In the same group, Ankole defeated Sebei 3-0 away in Kapchorwa. Abato Yusuf scored a brace before Paul Mucuurezi added the third.
In group C, Acholi defeated Karamoja 3-2 away in Moroto. Lokuna Emmanuel scored a double for Karamoja while Hudu Mulikyi, Otto Isaac and Asaba Richardson were on target for Acholi who stretched their lead to 12 points.
Bunyoro held West Nile 1-1 in Masindi. URA FC player Rothomio Cromwell gave the visitors the lead, unfortunately Brian Ahebwa pulled off an equaliser towards the end of the game.
Unbeaten Bukedi in group D made their way to the quarterfinal after a 1-1 draw away in Soroti against Teso Province. Ocen Ben scored an equaliser in the 70th minute after Egessa Vincent had put the home side infront in the 63rd minute.
Bukedi still leads the table with 11 points from 5 games.
In Kabale, last season top scorer James Alitho was on the mark with his strike on target in a 2-0 victory over Kigezi.
Richard Wandyaka and Hamis Tibita were on target for the visitors. Kikonyogo was named Man of the Match. Busoga midfielder Shabena Daniel was shown a red card plus his coach Charles Ayeko.